MICROSCOPICAL EXAMINATION OF BACTERIA IOI 



polar stain. Other bacteria when stained with methylene blue, 

 or similar stains, present granules of various sizes within the 

 cell protoplasm, usually in a polar or equatorial position. 

 These peculiar staining reactions may indicate a variation in 

 the chemical composition of the protoplasm, or the variations 

 may be merely physical ones. In Gram's stain the principle 

 involved is undoubtedly of a chemical nature. 



