CHAPTER VI 



CYTOLYSINS 



INTRODUCTION. 

 HEMOLYSINS. 



IMMUNE HETERO-HEMOLYSINS. 

 HEMOLYTIC AMBOCEPTORS. 



PREPARATION OF IMMUNE HEMOLYSINS. 

 OBTAINING ANTIGENIC BLOOD. 

 PREPARATION AND COLLECTION OF IMMUNE SERA. 

 TITRATION OF IMMUNE SERA. 

 TITRATION OF COMPLEMENT. 



QUANTITATIVE RELATIONS OF AMBOCEPTOR AND COMPLEMENT. 

 QUANTITATIVE RELATIONS OF AMBOCEPTOR AND ANTIGEN. 

 RELATIVE AFFINITIES OF AMBOCEPTOR AND COMPLEMENT. 



SELECTIVE ABSORPTION OF AMBOCEPTOR. 

 INFLUENCE OF AMOUNT OF COMPLEMENT. 

 RATE OF ABSORPTION OF AMBOCEPTOR. 

 DISSOCIATION OF AMBOCEPTOR ANTIGEN UNION. 

 SPECIFICITY OF AMBOCEPTORS. 



GROUP REACTIONS. 

 NATURE OF THE ANTIGEN. 

 NATURE OF THE AMBOCEPTOR. 



MECHANISM OF OPERATION OF AMBOCEPTOR. 



CONGLUTININS. 

 COMPLEMENT. 



DISTRIBUTION. 

 ALTERATIONS OF AMOUNT. 

 METHOD OF OBTAINING COMPLEMENT. 

 ORIGIN OF COMPLEMENT. 

 NATURE OF COMPLEMENT. 

 PRESERVATION. 



VARIABILITY OF COMPLEMENT. 

 MULTIPLICITY OF COMPLEMENTS. 

 COMPLEMENTOIDS. 

 COMPLEMENT FRACTIONS. 

 NORMAL HETERO-HEMOLYSINS. 



PROPORTIONS OF AMBOCEPTOR AND COMPLEMENT. 

 NORMAL ISO-HEMOLYSINS. 

 ANTI-AMBOCEPTORS. 

 ANTI-COMPLEMENTS. 

 PHYSICAL HEMOLYSIS. 



FRAGILITY OF ERYTHROCYTES. 

 CHEMICAL HEMOLYSIS. 

 BACTERIAL HEMOLYSINS. 



OTHER VEGETABLE HEMOLYSINS. 

 VENOM HEMOLYSINS. 

 CYTOTOXINS. 

 SPECIFICITY. 

 LENS CYTOTOXIN. 

 BACTERIOLYSINS. 



THE PFEIFFER PHENOMENON. 

 BACTERIOLYSIS IN VITRO. 

 WRIGHT'S METHODS. 

 NEISSER-WECHSBERG PHENOMENON. 

 BUXTON'S METHOD. 

 BIOSCOPIC METHOD. 



SUMMARY. 



US 



