PREPARATION OF MEDIA 89 



Water, 1000 cc. 



Glycerin, 30 to 40 grams 



Sodium chloride, 5 to 7 grams 



Calcium chloride, 0.1 gram 



Magnesium sulphate, 0.2 to 0.4 grams 



Dipotassium phosphate, 2.5 to 3 grams 



Ammonium lactate, 6 to 7 grams 



Sodium asparaginate, 3 to 4 grams 



Instead of this complicated solution, one may employ 

 many simpler ones; for example, such as is recommended by 

 Voges and C. Frankel, 1 which is as follows : 



Water 1 liter 



Sodium chloride 5 grams 



Neutral commercial sodium phosphate 2 grams 



Ammonium lactate 6 grams 



Asparagin 4 grams 



A substitute for Loeffler's bloodserum in the diagnosis 

 of diphtheria. Gorham recommends the following proteid- 

 free medium which in the liquid form had been found by 

 Hadley to be favorable for the growth and toxin production 

 of Bad. diphtheriae. It was used in combination with agar 

 as a substitute for Loeffler 's bloodserum. It is as follows : 

 Glycerin 3.40 parts 



Sodium chloride 0.60 " 



Calcium chloride 0.08 " 



Magnesium sulphate 0.32 " 



Dipotassium phosphate 0.23 " 



Ammonium lactate 0.75 " 



Ferric phosphate 0.08 " 



Glycocoll 0.10 " 



Distilled water to make 100 parts, 1.5 per cent agar, re- 

 action made +1 with sodium hydrate. 



It was found possible to make a diagnosis from swab- 

 bings on this medium in less than 15 hours. 



The organisms were of about the same types as on the 

 1 Voges and Frankel. Hyg. Rundschau, 1894, p. 769. 



