96 MICROBIOLOGY 



Dorset's egg medium. 1 The whole egg is preferable. 

 Carefully break the shell of the required number (3, 6, or 

 more) of fresh eggs, drop the entire contents into a sterile 

 beaker, and carefully stir with a sterile glass rod, care being 

 taken to avoid air bubbles. After the egg is well mixed it 

 is poured into test tubes (6 to 10 cc. in each) and sterilized 

 by heating, preferably in a serum water bath, at 70 C. for 

 from 4 to 5 hours each day for 2 days. After sterilization 

 the tubes should be sealed. Before using, add a few drops of 

 sterile water, or better perhaps, of 5% glycerin or of glucose 

 to afford sufficient moisture. This medium is used largely at 

 the present time for the cultivation of tubercle bacteria. 

 1 Dorset. Am. Med., April 5, 1902. 



