ISOLATION AND CULTIVATION 10T 



for the cultivation of anaerobic bacteria. Hunziker 2 has 

 reviewed the methods and illustrated most of the apparatus 

 suggested for studying this class of bacteria. 



Buchner's Method. Use two test tubes of different 

 sizes, drop into the large tube a small wire support on which 

 the small tube is subsequently placed. The small test tube 

 contains the inoculated medium and is closed with a loose 

 cotton plug. Put into the large test tube one gram of dry 

 pyrogallic acid and then ten cc. of a one-tenth solution of 

 potassium hydroxid (1 part liquor kali caust. to 10 parts 

 of water). Then quickly lower the small culture tube and 

 close the latter hermetically with a new, elastic, well fitting,, 

 paraffined rubber stopper. Shake well. Buchner observed 

 that the above stated amount of pyrogallic acid and potas- 

 sium hydroxid will completely absorb the oxygen in a tube 

 with a cubic content of 100 cc. in 24 hours at incubator tem- 

 perature. At lower temperature, as lor instance in the re- 

 frigerator, the absorption of oxygen is much slower. Fre- 

 quent shaking hastens the absorption. 



2 Hunziker. Jour, of Applied Microscopy and Laboratory Meth- 

 ods, Vol. V (1902) p. 1694. 



