130 



MICROBIOLOGY 



consider it an important aid in the differentiation of certain 

 bacteria. 



Paltauf (Proceedings N. Y. Pathological Society, Oct. 

 1909) recommends the use of a staining solution composed of 

 from 3 to 5 cc. of aniline oil, 90 cc. of distilled water and 7 cc. 

 absolute alcohol in place of aniline water. 



BACTERIA WHICH DO AND DO NOT STAIN ACCORDING TO GRAM. 



GRAM NEGATIVE 



Bact. abortionis 



aerogenes. 



cholerae gallinarum. 



cuniculicidum. 



pullorum. 



sanguinarium (?). 



suisepticum. 

 " mallei. 

 B. coli. 

 " enteritidis. 

 " dysenteriae group. 

 " of malignant oedema. 

 * l mucosus capsulatus. 

 11 oedematis. 

 " proteus. 

 <; suipestifer. 

 " typhosus group. 

 B. vulgar is. ^ 

 Ps. pyocyaneus. 

 Spirilla cholerae and allied 

 forms. 



Gram-Weigert 1 method of staining bacteria. This is a 

 valuable stain for Gram positive bacteria in sections. The 

 sections are stained 4 to 5 minutes in lithium carmine* De- 

 hydrate in 95% alcohol, stain in aniline-gentian violet for 10 

 to 15 minutes, wash in tap water and place sections in Lu- 



GRAM POSITIVE 



Str. of apoplectiform septi- 



cemia. 



Str. capsulatus gallinarum. 

 Micrococcus pyogenes. 

 Bact. acidi lactici. 



44 anthracis. 



" astheniae. 



44 bulgaricus. 



" caseous lymphadenitis. 



<4 diphtheriae group. 



" of leprosy. 



" rhusiopathiae. 



41 subviscorum. 



*' truttae. 



'" tuberculosis. 

 B. alvei. 

 B. tetani. 

 B. Chauvaei. 

 B, subtilis. 



1 Weigert. Fort, de Med., Bd. V (1887) p. 228. 

 * Formula for lithium carmine: 



Carmine 3.5 grams. 



Sat. aqueous solution lithium carbonate 100 cc. 



Thymol a small crystal for preservation. 



