240 MICROBIOLOGY 



solution as sent out,* and make the injection beneath the skin 

 of the middle of one side of the neck, where the local swelling- 

 can be easily detected. 



"Carefully sterilize the syringe after injecting each horse 

 by flaming the needle over an alcohol lamp or, better, use 

 separate syringes for healthy and suspected animals. If the 

 same syringe is used, inject the healthy animals first and flame 

 the needle of the syringe after each injection. 



' ' Take the temperature every two hours for at least 18 

 hours after the injection. Sterilize the thermometer in a 5% 

 solution of carbolic acid, or a 0.2% solution of corrosive 

 sublimate, after taking the temperature of each animal. 



: ' The temperature as a rule, will begin to rise from four 

 to eight hours after the injection, and reach its maximum 

 from ten to sixteen hours after injection. On the day suc- 

 ceeding the injection take the temperature at least three times. 



1 ' In addition to the febrile reaction, note the size, appear- 

 ance, and duration of any local swelling at the point of injec- 

 tion. Note the general condition and symptoms of the animal,, 

 both before, during, and after the test. 



"Keep the solution in the sealed bottle and in a cool 

 place, and do not use it if it is clouded or if it is more than 

 six weeks old. When it leaves the laboratory of the Bureau it 

 is sterile." 



Luedmersen and Glenny 4 found after a careful investiga- 

 tion of this subject that a marked local swelling following the 

 injection of mallei n occurred in horses immunized to other 

 bacterial products. This is important in considering the diag- 

 nostic value of local swellings where the. horses have been 

 immunized against other bacteria such as diphtheria. 



Agglutination. The agglutination method was first ap- 

 plied to glanders by M'Padyean. 5 Later it was recommended 



'MMallein prepared and distributed by the Bureau of Animal 

 Industry. 



4 Leudmersen and Glenny. The Jour, of Hygiene, Vol. VIII 

 (1908)- p. 36. 



5 M'Fadyean. Jour. Comp. Path, and Therap., Vol. IX (1896) p. 

 322. 



