GENUS BACTERIUM 2L43L 



for the control of glanders by Schiitz and Miessner, 6 and also 

 Schnurer 7 reccmmended this method for the diagnosis of 

 glanders. 



The method as formulated by Schiitz and Miessner for 

 the eradication of glanders is as follows : s 



Twenty to 50 cc. of blood are taken from the glandered or 

 suspected horse, the date and history of the case being recorded 

 and sent to the laboratory. 



All horses whose blood agglutinates in dilutions of 1-1,000 

 or higher should be destroyed. 



In the same way, all horses should be destroyed whose 

 blood agglutinates in dilutions of only 1-500 to 1-800, if they 

 show symptoms of glanders. 



All other horses in which the agglutination is 1-500 to 

 1-800 should be isolated and destroyed only when justified by 

 a second test, in which the maximum dilution for agglutination 

 is changed; on the other hand, they may be pronounced free 

 from glanders if at the second test the dilution remains un- 

 changed. 



After glanders is established, the blood of horses in the 

 same stable should be tested after three weeks ; and this should 

 be repeated until the last two tests show in all horses indi- 

 vidually a uniform, reaction. 



In cur work we have applied the general method recom- 

 mended by Schiitz and Miessner, with such slight modifica- 

 tions as seemed desirable. The method for the routine diag- 

 nosis which we have found to be very satisfactory is as follows : 



Culture. As pointed out by Schiitz and Miessner, all cultures of 

 B. mallei do not agglutinate satisfactorily. It was also shown by 

 their work that a suitable culture when obtained is liable, at unex- 

 pected intervals, to lose its responsiveness to the agglutinin. This can 

 be prevented by passing the organisms through a guinea pig at least 

 once in three weeks. The organisms were grown for from 48 to 72 

 hours on acid-glycerin agar (5 per cent glycerin and with a reaction 



6 Schiitz and Miessner. Archiv. f. wis. u. prak. Thierheilk., Bd. 

 XXXI (1905) p. 353. 



7 Schnurer. Centralbl. f. Bakt, Bd. XXXIX (1905) p. 180. 



8 Moore and Taylor. The Jour, of Inf. Diseases, 1907, Sup. No. 

 3, p. 85. 



