GENUS BACILLUS 341 



Serum. On blood serum the growth is vigorous. The 

 serum is rapidly liquefied with the production of gas. 



Gelatin. The medium is liquefied. 



Potato. Potato was used by Gaffky. The organism grows 

 readily upon it. 



Bouillon. In bouillon there is a uniform clouding of the 

 liquid and a granular sediment is formed. No pellicle appears. 



Milk. Milk is slowly coagulated. 



Life conditions and properties. This organism requires 

 for its development the absence of atmospheric oxygen. It 

 develops at a temperature between 20 to 37 C. It produces 

 gas with a peculiar disagreeable odor in all media containing 

 sugars. The spores are very resistant, which is the explana- 

 tion for the soil's being so extensively infected. 



Pathogenesis. It is pathogenic for mice, guinea pigs, and 

 rabbits. Horses, dogs, goats, sheep, pigs and chickens are 

 susceptible. It is also virulent for man. Inoculated subcu- 

 taneously it causes an extensive hemorrhagic oedema of the 

 subcutaneous tissue. It does not produce an odor like black 

 leg and there is little if any gas. Animals become infected 

 from the soil through wounds. This disease is confined 

 naturally very largely to domesticated animals. It is stated 

 that animals that recover from malignant oedema are subse- 

 quently immune to the disease. 4 



BACILLUS LACTIMORBI JORDAN AND HARRIS. 



Place in nature. This organism was found by Jordan 

 and Harris 3 to be associated with the lesions in cattle that 

 died of milk sickness or "trembles." It was thought to be 

 the cause of this somewhat serious affection. They report 

 finding it or an organism not distinguishable from it in the 

 soil of regions where milk sickness has not occurred. They 

 also found it but not in large numbers in the soil in localities 

 where milk sickness appears, in normal cows ' f eces, on various 



4 Arloing and Chauveau. Ann. de medecine vet., 1884. 

 Jordan and Harris. Jour, of Inf. Diseases, Vol. VI (1909) p. 

 401. 



