SERUM DIAGNOSIS 



467 



one to show that the complement alone will not produce 

 hemolysis (without the amboceptor), the second that the am- 

 boceptor alone without the complement will not produce 

 hemolysis, and the third that the salt solution alone will not 

 produce hemolysis. Thus in the first control tube we add 3.5 

 cc. of salt solution, 0.5 cc. of complement, and 1 cc. suspension 

 of sheep corpuscles. In the second control tube we add 3 cc. 

 salt solution, 1 cc. of the 1-100 dilution of the amboceptor and 

 1 cc. suspension of sheep corpuscles. In the third control tube 

 we add 4 cc. salt solution and 1 cc. sheep corpuscles. A test 

 tube rack with two rows of holes is very convenient for holding 

 the tubes for these tests. 



TABLE NO. II. 



TITRATION OF RABBIT SERUM (HEMOLYTIC AMBOCEPTOR). 



a. 0.9% NaCl solution. 



b. 0.5 cc. of a 10% solution of the complement. 



c. 5% suspension of washed sheep corpuscles in salt solution. 



