474 



MICROBIOLOGY 



The amount of these immune bodies in the blood of glandered 

 horses is not uniform and probably depends to some extent on 

 the degree of infection present, therefore it is advisable to 

 use in the test such quantities of the serum as will prove 

 sufficient for the reaction to take place. 



TABLE NO. VI. 



THE FINA1, TEST FOR GLANDERS. 



1. 0.9 per cent NaCl solution. 



2. Suspected horse serum to be inactivated for 30 minutes at 

 56-57 C. in water bath, in order to destroy the complement which 

 is present in the serum of every horse. 



3. One-half of the quantity which does not prevent hemolysis and 

 established by titration (See Table V). 



4. The determined smallest quantity established according to 

 the preliminary test (See Table III). 



5. Double the quantity previously determined by titration (See 

 Table II). 



6. 5% suspension of washed sheep blood corpuscles. 



* Place for 1 hour in incubator or one-half hour in water bath 

 at 37 C. 



