476 MICROBIOLOGY ; 



for 1 hour (or water bath at 37 C. for y 2 hour) . This is done 

 in order to allow the union or fixing of the complement which 

 will become locked up with the antigen and the bacteriolytic 

 amboceptor in case the suspected serum came from a glandered 



Complement 



Hemolytic ambo- (Guinea pig) 



ceptor (rabbi t)/*^ 



Red blood cell Union of red blood Hemolusis *ilt result 



(Sheep) cell and amboceptor 



(no hemolysis] 



<%&&* <"L> 



\ofbacter- 

 BaTteria 'lr"no Ba^o^) Bactoly3* ill result 



Red blood cell 



hemolytic 



Complement QflM&flJ X^X amboceptor 



Ho Bactenolusts 

 Bacteria (Gland* 

 ers antigen) Hemo/ysis will result 



Horse Serum 



with ganders * Red blood cell 



Hemolyhc ^-^^ 



Bacteria (q/and- Bacteriolysis am ocep , nohemolijsis will result 



ers antigen) 



A -Hemolytic System 



B -Bacteriolytic System 



C -negative reaction, normal 'horse serum 



D -Positive reaction^ a,/andered horse serum 



0/agrammatic Representation of Complement F'/xafion?' 



Fig. 101. A scheme illustrating the complement fixation method de- 

 vised by Noguchi and modified slightly by Mohler and Eichhorn 

 for diagnosis of glanders. 



animal. If. the bacteriolytic amboceptor is not present or the 

 suspected serum was from a healthy animal the complement 

 will not become locked up or fixed. 



