278 Kqjima 



No. 12. — Suffered from slight diarrhcea from the third day of 

 feedino-, and appetite somewliat affected. At the end of the feeding 

 it had lost ]5 grm. in weight. Killed after two days' intermission 

 of feeding. 



No. 13. — Diarrhoea from third day onwards, with decreased 

 appetite. Killed three days after cessation of thyroid feeding. 



Nos. 14 and 15. — Animals suffered from diarrhcea from the fourth 

 day of feeding. Their appetite also decreased, and they were com- 

 paratively inactive. No. 14 was killed after five da^^s, and No. 15 

 after six days of the thyroid feeding. Their weights then were 

 164 grm. and 193 grm. respectively. 



In all these animals the intestines, both small and large, show 

 congestion. There are no other noticeable changes post-mortem. 



Pancreas. Nos. 7 and 8 (one and two days' thyroid 

 feedino-). — There is as yet no evidence of karyokinesis. The 

 zymogen granules are scanty, but otherwise there is no difierence 

 from the normal, except that No. 8 (two daj's' feeding) already 

 begins to show the marked vacuolation of some of the alveolar 

 cells which is characteristic of thyroid feeding. 



No. 9 (three daj's' thyroid feeding). — Both the alveoli and 

 the cells vary more in size than in the normal, and this is also the 

 case with the cell-nuclei, which measure from 3/x to 9^. A few are 

 of still larger size (10/x), and many are more deeply stained than 

 normal. Some show enlarged nucleoli. Both nuclei and nucleoli 

 are stained blue by Mallory. The nuclei have abundant chromatin 

 granules. A few cells show typical mitoses. The zymogen granules 

 are scanty in all the alveoli. Many of the cells exhibit vacuoles. 



Nos. 10-15 (four to six days' thyroid feeding). — The alveoli 

 are smaller than normal, and contain many small cells. A consider- 

 able number of the alveolar cells are in mitosis. These cells are 

 relatively large. The mitoses are much more abundant in the rats 

 which have been fed for five and six days than in the four-day 

 animals. The nuclei of the cells vary considerably in size (3^ to 9fx). 

 Some of the large nuclei contain very distinct nucleoli staining 

 deeply with hgematoxylin. Both nuclei and nucleoli stain blue by 

 Mallor3^ The zymogen granules are scanty throughout. Many of 

 the alveolar cells exhibit vacuoles of varying size. Small vacuoles 

 are observable in the islet-cells. 



Summar3^ — In all the animals of this series (like those animals of the 

 last series in which fresh sheep's thyroid was also given) the usual effects 

 of thyroid feeding are observed. The zymogen granules are considerably 

 diminished; more so than in animals dieted with dry ox-thyroid. This 

 chanofe is seen even after a sino-le dose, whilst vacuolation of the cells is 

 visible after two days' feeding. 



Mitotic figures began to appear after three days' feeding ; their number 

 gradually increased up to six days. 



Most of the animals suffered from diarrhoea, and their appetite 

 decreased after three days' feeding ; the weights in consequence diminished 

 throughout. 



