300 Kojima 



Pancreas. — In the rats which were fed for five days (Nos. 66 

 and 67) many of the cells are abnormally small, with comparatively 

 large nuclei and a diminution of zymogen granules (figs. 20-22). The 

 larger nuclei, many of which measure as much as from 9/x to 11/x, 

 contain large nucleoli stained red by Mallory. The smaller cells 

 contain numerous granules which are finer than the usual zymogen 

 granules ; they may represent a pro-zymogen. These cells have the 

 cytoplasm stained light red instead of blue by Mallory. There is no 

 evidence of mitosis nor of vacuolation. 



In the rats which were fed for three and four days respectively 

 (Nos. 64 and 65) the pancreas shows much the same microscopic 

 appearance as the normal, with fewer large nuclei. There is no evi- 

 dence of mitosis : some of the small cells are vacuolated. Zymogen 

 granules are abundant in all the alveoli. 



Sodium Bromide, Sodium Chloride, and Sodium Fluoride. 



Similar experiments were made with these substances, an equal dose, 

 viz. O'l grm. per rat per diem, being administered during five days. Sodium 

 bromide and sodium chloride produced no appreciable symptom. Sodium 

 fluoride caused considerable effect upon the appetite ; one of the two 

 animals to which it was administered died on the fourth day. The other 

 survived the experiment, and was killed along with the rest after five days. 



Post-mortem. — In the two rats fed with an addition of sodium fluoride 

 there was general congestion of the intestines. In No. 77, the rat which 

 survived the administration of sodium fluoride, the stomach was found to 

 be contracted and to contain a thick mucous fluid and but little food. The 

 contents of the small and large intestines were dark and watery ; the 

 intestines were congested but showed no ulceration. The liver and spleen 

 were also congested. The pancreas appeared small, and pale in colour. The 

 other animals show nothing noticeable. 



Pancreas. — The microscopic appearances are almost normal, but 

 in Nos. 72, 73, 74, and 75, which were fed with addition of sodium 

 bromide and sodium chloride, a few cells show mitoses, and one or 

 two such were found in the section of the pancreas of No. 77 (sodium 

 fluoride). Zymogen granules are plentiful in all the alveoli, and 

 exhibit no difference from the normal. There is no vacuolation to be 

 seen in the cells, nor anything to record about the islets. 



Sodium Carbonate, Sodium Phosphate, and 

 Sodium Sulphate. 



Doses similar in amount to the salts already described were adminis- 

 tered to a series of rats, and when these doses produced no obvious result, 

 double doses were subsequently administered. 



Pancreas. — The effect of doubling the dose was to produce 

 within the pancreas the appearance of a few mitoses, but no changes 

 at all were apparent in the amount of zymogen, nor was there any 



