Studies on the Endocrine Glands 303 



seen. There is no evidence of mitosis. Zymogen granules are far 

 less numerous in all the alveoli than is the case in the normal 

 pancreas. A few nuclei are larger than the rest, and some show a 

 tendency to stain yellow instead of blue by Mallory. Some of the 

 cells ai-e vacuolated. The islets exhibit no appreciable change. 



Pituitary Body. 



The preparations of the pituitary body employed were (1) fresh ox- 

 pituitaries, which were separated into anterior and posterior lobes, dried in 

 an incubator at 37" C, and ground to a fine powder in a clean mortar. 

 The powder was kept in an exsiccator. (2) Ox-pituitaries which had been 

 preserved for a considerable time in chloroform. These were similarly 

 separated into anterior and posterior parts, which were ground and pre- 

 served apart from the others. 



(a) Feeding with Anterior Lobe. 



Four female rats (Xos. 96, 97, 98, 99), of weights varying from 160 grm. 

 to 265 ofrm., were fed durincf a week with 0-3 grm. of dried anterior lobe. 

 In one group of two the preparation from the fresh gland was used, in the 

 other group the preparation from the chloroform-preserved gland. Alto- 

 gether, during the period of feeding each animal of Nos. 96 and 97 

 received 2-1 grm. during seven days, corresponding to about 10 grm. of 

 fresh, undried anterior lobe. The animals showed no special symptoms 

 when killed, after seven days, and their weights differed very little from 

 those at the commencement. To the second group of two rats, Nos. 98 and 

 99, a little more of the anterior lobe of pituitary body (that which had 

 been preserved in chloroform) was given, and this feeding was continued 

 for six days. The total amount which they received also corresponded to 

 the anterior lobes of about ten fresh ox-pituitaries. In a third experiment 

 as much as 1 grm. of the dried anterior lobe (chloroform-preserved) was 

 given per rat per diem (rats Nos. 100 and 101, weights 180 grm. and 

 190 grm. respectively). This treatment was continued for one week. No 

 special symptoms were obvious, and at the end of the time the weights had 

 not altered. 



Post-mortem there is nothing noticeable macroscopically. 



Pancreas. — The pancreas shows certain changes on microscopic 

 examination. Thus the alveoli and alveolar cells are smaller than 

 normal. The outer zone of each cell is relatively broader. There 

 are quite a number of fairly large nuclei (7'5|a) containing large 

 nucleoli staining red by Mallory. Zymogen granules are far scarcer 

 than normal. There is no difference of appearance noticeable in 

 the islets. 



(b) Feeding with Posterior Lobe. 



For this purpose dry posterior lobe of ox-pituitary was given to two 

 groups of rats, that for the one group being derived from the fresh gland 

 VOL. XL, NOS. 3 AND 4. — 1917. 20 



