304 



Kojima 



and that for the other from the chloroform-preserved gland. In the 

 former case 0*02 grm. and in the latter case 0'03 grm. of dry posterior 

 lobe were given per rat per diem. The feeding was continued in each 

 case for a week. No special symptoms were observed. The weights at 

 the end of the experiment were increased in some of the rats and 

 diminished in others, but not to any great extent. The amount of 

 posterior lobe given per rat during the week corresponds to about ten 

 posterior lobes of fresh ox-pituitaries. 



This experiment was repeated on two other groups of two rats, 0'03 

 grm. of dry posterior lobe of ox-pituitary being administered to each — in 

 the one case derived from the fresh glands, and in the other from the 

 glands preserved in chloroform. The administration was continued for a 

 week ; the weights at the end showed very little change. The object of 

 repeating the experiment was to observe the effect, if any, upon the amount 

 of urine, which I had omitted to notice in the preceding experiment. In 

 these rats the amount of urine was greatly increased, the increase being 

 much more marked in the animals to which the posterior lobe from fresh 

 pituitary was administered than in those which received posterior lobe 

 substance of glands preserved in chloroform : in these there was a little 

 more increase in weight. The following table gives the quantities of urine 

 passed in these animals, which were all males: — 



It should be mentioned that the animals were freely supplied with water, 

 besides that which they received along with the rusks, the water being 

 offered from time to time but never left in the metabolism ca^e. In the 

 urine of the rats which were fed with 0-03 grm. of dry posterior lobe 

 (fresh), Nos. 113 and 114, there was a trace of glucose, as evidenced by 



