]42 POULTRY DISEASES 



tory to isolate any germ which might have been the cause of the 

 disease were unsuccessful. 



The second flock was from eggs that had been produced by birds 

 in which roup had appeared the preceding winter. Five birds 

 about four months old were sent to the laboratory for study with 

 the following history: The birds had good hygienic surroundings, 

 were moved from place to place, given fresh water and good quality 

 of feed, with plenty of green stuff, but without success; the birds 

 not only did not thrive, but continued to lose flesh and finally died, 

 notwithstanding that most of them had a ravenous appetite. 



Chick No. 3. — Hemoglobin. 65 per cent: erythrocytes, 2,920,000; 

 leukocytes, 28,000. Differential count: polymorphonuclear neutro- 

 philes,' 39 per cent; eosinophiles, 30 per cent; lymphocytes, 29 per 

 cent; mast cells, 2 per cent. 



Chick No. 4. — Hemoglobin, 65 per cent: erythrocytes, 2,600.000; 

 leukocytes, 14,000. Differential count: eosinophiles, 31 per cent; 

 mononuclears, 8 per cent; lymphocytes, 60 per cent; basophiles, 1 

 per cent. 



Chick No. 5. — Hemoglobin, 75 per cent; leukocytes, 34,000; eryth- 

 rocytes, 3,000,000. Differential count: polymorphonuclear neutro- 

 philes, 4 per cent; eosinophiles. 50 per cent; basophiles, 3 per cent; 

 mononuclears, 4 per cent; lymphocytes, 39 per cent. 



Chick No. 6. — This bird was about four months old, stunted in 

 growth, "going light," and had contracted roup from another bird 

 The blood study shows the following: hemoglobin, 60 per cent; 

 leukocytes, 16,000; erythrocytes, 3,600,000: thrombocytes, 436.000. 

 Differential count: eosinophiles, 3 per cent; mononuclears, 4 per 

 cent: lymphocytes, 89 per cent; mast cells, 4 per cent. 



All efforts to isolate germs from the liver, spleen, kidneys and 

 heart-blood were unsuccessful. 



More study must be done on this disease to determine the true 

 cause, before rational treatment can be outlined. 



Treatment. — Best results, in flocks of young birds, have been 

 obtained by giving one tablespoonful of Epsom salts to each 

 12 birds. Dissolve the Epsom salts in water and use this 

 water to mix with bran or mash. Repeat twice weekly. See 

 that the flock has plenty of shade and clean, pure water at all 

 times. 



The birds should receive one-fourth to one grain ferrous 

 sulphate once a dav in soft feed. The prognosis is not lio{)e- 

 ful. 



With our present knowledge we will look to sanitary sur- 

 roundings for the control of this condition — clean coops and 

 yards, good feed and water. A tablespoonful of ]io\vdered 

 ginger to each dozen hens may be given with advantage once 

 or twice a day in soft feed. 



In going light in addition to a cathartic of Epsom salt the 

 following given once daily has yielded fair results: calcium 

 carbonate 120 grains, magnesium sulphate 240 grains, sodium 

 bicar])onate 360 grains, sodium chlorid 60 grains, sodium sul- 

 pliate 120 grains. This is one dose for 120 birds. 



