THE METHODS OF CULTIVATING MICROBES 53 



using Woodhead's storage flasks, which have already 

 been described. For the inoculation of various 

 media, pieces of platinum wire, either mounted in 

 sealed glass-tubing, or unmounted, are used. They 

 are sterilised by being heated in the Bunsen flame. 

 To inoculate any medium, the sterilised needle, or a 

 capillary pipette (Klein), is first dipped into the 



A. :B. 



-JT 



FIG. 20. INJECTION SYRINGES. 

 (A, Dr. Petri's. B, Dr. Klein's. C, Dr. Koch's.) 



inoculating substance, and then transferred to the 

 medium. Where Aitken's tubes and similar devices 

 are used the medium contained therein is inoculated 

 by using unmounted sterilised needles. These are 

 dipped into the inoculating substance and then 

 dropped into the fluid medium. To inoculate 



