THE METHODS OF CULTIVATING MICROBES 55 



Solid egg albumin is sometimes used as a cultiva- 

 tion medium. The white of an egg is poured on to 

 a slab of glass (sterilised), where it is coagulated and 

 sterilised by being heated in the steam steriliser, 

 or in the hot-air steriliser if tho temperature be 

 properly regulated. Solid egg albumin (Fig. 21) is 



FIG. 21. MICROCOCCUS CHLORINUS. 



Growing on sterilised white of egg, after a fourth attenuation. 

 (The white of egg coagulated and sterilised upon a slab of blackened glass.) 



readily inoculated by means of a platinum needle 

 containing the inoculating material. Micrococcus 

 chlorinus grows very well on this medium. 



Dr. F. Hueppe's method of cultivating on egg 

 albumin is different from the above. It is as 

 follows: The shell is first disinfected with a solu- 



