70 A MANUAL OF BACTERIOLOGY 



(6) Eanvier's picro-carmine stain contains 1 gramme 

 of carmine, 3 cc. of ammonia, 10 cc. of distilled 

 water, and 200 cc. of a cold, saturated solution of 

 picric acid ; (7) vesuvin stains are prepared by dis- 

 solving 3, 4, or 5 grammes of vesuvin in 100 cc. of 

 distilled water ; (8) Dr. Gibbes' solution for double 

 staining contains 2 grammes of magenta and 1 

 gramme of methyl violet, which are triturated in a 

 glass mortar with 15 cc. of alcohol (in which has 

 been dissolved 3 cc. of aniline oil). To this mixture 

 is added 15 cc. of distilled water; (9) Gram's iodine 

 solution is prepared by dissolving 1 gramme of 

 iodine and 2 grammes of potassium iodide in 300 

 grammes of distilled water; (10) LofHer's stain con- 

 tains 30 cc. of a concentrated alcoholic solution of 

 methylene blue, and 100 cc. of an aqueous solution 

 of potassium hydroxide (1 in 10,000) ; (11) an eosin 

 solution is prepared by dissolving 5 grammes of 

 eosin in 100 cc. of distilled water. 



We now continue the description for staining 

 microbes and tissues. To stain tissues containing 

 microbes, place them in either an aqueous solution 

 of methyl violet (2 -2 5 grammes in 100 cc. of water), 

 or one of gentian violet (containing the same 

 strength of solution), and allow them to remain in 

 the solution for some hours. When deeply stained, 

 wash in water to remove the excess of the stain, 

 and then lay them out flat in methylated spirit, and 

 let them remain until no more colour comes away. 

 Transfer them to absolute alcohol, and then oil of 

 cloves, and mount in Canada balsam (Gibbes). 



To double stain bacilli which produce spores, the 



