80 A MANUAL OF BACTERIOLOGY 



solution of ammonium carbonate (1 per cent.). To 

 demonstrate the spores of this bacillus, cover-glass 

 preparations and tissue-sections must be placed 

 in a hot solution of fuchsine. They are then 

 decolourised with nitric acid (see Ehrlich's method), 

 after-stained with methylene blue, and mounted as 

 usual, after dehydration and clarification in the 

 media already described. 



The most important methods for staining the 

 Micrococcus pneumonice are as follows: (1) By the 

 method of Gram. (2) Cover-glass preparations of 

 pneumonic sputum and exudations are treated 

 with acetic acid, stained with gentian violet, and 

 temporarily mounted in distilled water, or water 

 and glycerine, i.e. for immediate examination; or 

 they may be dried and permanently mounted in 

 Canada balsam. 



Cover-glass preparations of gonorrhceal pus, 

 blood, or of artificial cultivations of the Micrococcus 

 gonorrhcece are readily stained with an aqueous 

 solution of fuchsine. This method may be also 

 used for demonstrating the presence of the same 

 micrococcus in the tears of new-born infants suffer- 

 ing from purulent ophthalmia of gonorrhoeal origin. 



The cholera bacillus (Bacillus cholerce Asiaticce) is 

 stained by the following methods : (1.) The dis- 

 charges, etc.. containing the microbe are spread and 

 dried on a cover-glass. They are then stained with 

 an aqueous solution of fuchsine, washed with water, 

 dried, and mounted in Canada balsam. (2.) The 

 hardened sections x of the intestines are placed for 



1 Hardened in absolute alcohol. 



