82 A MANUAL OF BACTERIOLOGY 



(i.e. containing the Micrococcus scarlatince) are stained 

 with a saturated solution of methyl violet. The 

 micrococci, adhering to the scales of the desqua- 

 mating epidermis in such cases, are also stained 

 with the same dye. As the scarlatina micrococcus 

 has been found in diseased cow's milk, 1 such milk 

 should be treated by the method described for the 

 examination of tuberculous milk (see p. 75). 



Bacillus lutyricus is best stained with a solution 

 of iodine in potassium iodide. 



Actinomyces is usually stained by Plant's method. 

 Sections of nodules, tumours, etc. (from cases of 

 Actinomycosis) are immersed for ten or twelve 

 minutes in a stain containing two grammes of 

 magenta, 3 cc. of aniline oil, 20 cc. of alcohol 

 (sp. gr. 0*83), and 20 cc. of distilled water. The 

 stain (with the sections) is warmed to 45 C. The 

 sections are rinsed in water, and after- stained in a 

 strong alcoholic solution of picric acid for about 

 eight minutes. They are then immersed in water 

 for five minutes, in alcohol (50 per cent.) for fifteen 

 minutes ; and finally passed through absolute alcohol 

 and oil of cloves, and mounted in Canada balsam. 

 The tissues containing this fungus may be examined 

 in the fresh state. A little of the tissue, etc., is 

 transferred to a microscopic slide, teased out with 

 needles, and then temporarily mounted in a drop of 

 glycerine and water. 



We have given most of the principal methods 

 for the examination of microbes. It may be re- 



1 See Dr. Klein's Reports to the Local Government Board, 

 1885-8. 



