158 A MANUAL OF BACTERIOLOGY 



cultivated at the temperature of the body. Spore- 

 formation takes place at the ends of the cells. 



MM. Arloing, Cornevin, and Thomas l have shown 

 that the virus is capable of giving immunity to 

 animals inoculated with it. The following are the 

 chief facts observed by them : (a.) Injection of a 

 very small quantity of the virus into the loose con- 

 nective tissue of any part of the body produces a 

 temporary illness, and protects the animals, (b.) 

 Injection of a moderate quantity into the scanty 

 connective tissue of the tail produces a slight affec- 

 tion, and confers immunity. Very large doses, 

 however, may cause death. A moderate quantity 

 injected into the cellular tissue in other parts of 

 the body causes death, (c.) Injection into the veins 

 does not kill, but confers immunity, and the same 

 result follows injection into the respiratory tract. 

 (d.) Cultivation does not deprive the microbe of its 

 virulence, but heating the spores to 85 C. for six 

 hours destroys their virulence. 



On page 117 of their book (loc. cit.), MM. Arloing, 

 Cornevin, and Thomas state that the following sub- 

 stances destroy or do not destroy the virulence of 

 this microbe : 



Do not destroy the virulence. 



Destroy the virulence. 



Alcohol (90 %). 



Glycerol. 



Sulphate of quinine (10 %) 



Hydrogen peroxide. 



Sodium hyposulphite. 



Ammonia. 



Tannic acid (20 %). 



Salicylic acid (O'l %). 

 Carbolic acid (2 %). 

 Boric acid (20 %). 

 Sodium salicylate (20 %). 

 Potassium permanganate (5 / ). 

 Mercuric chloride (01 %). 

 Silver nitrate (O'l %). 



Du Charbon Bacttrien (1883). 



