182 A MANUAL OF BACTERIOLOGY 



Dowdeswell, 1 the microbe appears to be a micro- 

 coccus, and it has been observed in microscopical 

 sections of the spinal cord of animals dead of 

 rabies. Dr. Fol's preparations were made by 

 hardening the spinal cord or brain by immersion, 

 directly after death, in a solution containing 2-5 

 grammes of potassium bichromate, and 1 gramme of 

 copper sulphate in 100 cc. of .water. The piece of 

 tissue is divided so as to be able to take up Weigert's 



FIG. 40. MICROCOCCI IN HYDROPHOBIA. 



A, In cerebral matter (after Fol). B, In human saliva. 



x 1000 



solution of hsematoxylin ; then placed in absolute 

 alcohol, imbedded in paraffin, and cut into sections 

 not more than -^Q mm. in thickness. The sections 

 are finally decolorised by a solution containing 2*5 

 grammes of potassium ferrocyanide, 2 grammes of 

 borax in 100 cc. of water. In these sections, Fol 

 found small micrococci (0*2 //, in diarn.) in the 

 lymph spaces of the neuroglia, and between the 



1 Journ. Roy. Microscop. Society, 1886 ; and Lancet, 1886. 



