200 A MANUAL OF BACTERIOLOGY 



Griffini and Cambria 1 observed the same micro- 

 cocci in the blood of pneumonic patients. Salvioli 

 and Zaslein 2 cultivated these microbes, derived from 

 the same source, in bouillon at 37 to 39 C. ; and 

 when injected into mice and rabbits they gave rise 

 to pneumonia. Giles 3 found the same microbes in 

 many cases of pneumonia in India ; and pure culti- 

 vations, when injected into the subcutaneous tissues 

 of rabbits, produced the disease. Those researches 

 have been confirmed by Afanassiew. 



When the artificially - cultivated microbe is 

 inoculated in the tissue of the lungs it produces 

 in animals all the characteristic symptoms of 

 pneumonia; the lungs become red, solid, and en- 

 larged, and pieces of them sink in water. In 

 pneumonia the blood is considerably altered, for 

 the hsematin, globulin, and the salts are greatly 

 reduced. 



According to Emmerich, the growth of the 

 micrococcus of pleuro-pneumonia on peptonised 

 gelatine is similar to the one derived from human 

 pneumonic sputum; and when this microbe is 

 injected into rabbits it produces typical pneumonia. 

 Nolen and Poels 5 injected pure cultivations of the 

 microbe of human pneumonia into cattle, and pro- 

 duced pleuro-pneumonia with all its characteristic 

 symptoms. However, it may be mentioned that 



1 Gentralblatt fur d. Med. Wissemch., 1883. 



2 Ibid., 1883. 



3 British Medical Journal, 1883. 



4 Comptes Rendus de la Socidte de Biologie (Paris), t. 5. 



5 Centralblattfur d. ;Med. Wissensch., 1884. 



