238 A MANUAL OF BACTERIOLOGY 



pure cultivations of the microbe. This substance is 

 said to have produced toxic effects when injected 

 into animals. ' These observers, however, did not 

 separate from the albumoses that were formed any 

 enzymes that might be present, consequently they 

 were working with a mixture of substances. The 

 products that they obtained gave most of the re- 

 actions of albumoses ; they were certainly toxic, 

 but they probably contained both enzymes and 

 albumoses' (Woodhead). 



B. diphtherice (No. 2), which is identical with 

 those of Koux and Yersin, Zarniko, Escherich, and 

 Loffler, acts very virulently on guinea-pigs on sub- 

 cutaneous inoculation : at the seat of the injection 

 a tumour is produced, which in its pathology and 

 in microscopic sections, completely resembles the 

 diphtheritic tissue of the human subject. In 

 human diphtheria B. diphtherias is present only in 

 the diphtheritic membrane, but neither in the 

 blood nor in the diseased viscera ; the same holds 

 good of the experimental guinea-pigs. In sub- 

 cutaneous inoculation with artificial culture, though 

 it causes in these animals acute disease and death 

 the lungs, intestine, and kidney are greatly con- 

 gested the diphtheria bacillus remains limited to 

 the seat of inoculation (Klein). 



Klein has shown that this microbe also attacks 

 the cat and cows, as well as man and the guinea- 

 pig. But, unlike human diphtheria, the disease 

 locates itself in the lungs of the cat (Fig. 47), i.e. 

 the lung is the organ in which the diphtheritic 

 process in the cat has its seat. The domestic cat 



