APPENDIX 335 



or weeks, a brownish-yellow colour is developed, and 

 ultimately the pellicle assumes the appearance of small 

 liquid drops. 



This microbe also grows on agar-agar, nutrient 

 gelatine, potatoes, and in bouillon. It measures from 

 1*5 to 2'5 fjb in length and 0'5 p in breadth, and forms 

 spores. According to Scheuerlein, 1 the same microbe 

 is present in most malignant growths (especially sar- 

 coma and carcinoma), and when pure cultures of it 

 were injected into the mammary glands of bitches, they 

 gave rise to small soft tumours. On no occasion has 

 Scheuerlein failed to produce these tumours, con- 

 sequently he believes that he has discovered the true 

 factor in the astiology of cancer. It may be remarked, 

 en passant, that Domingos Freire 2 has confirmed the 

 results of Scheuerlein ; but there is no doubt that they 

 still require further confirmation. 



VI. THE MICROBE OF WHOOPING COUGH. 



Afanassieff 3 has found a bacillus in the pearly 

 phlegm of persons suffering from whooping cough. 

 This microbe, which measures from 0*6 to 2'2 /z, in 

 length, is readily cultivated on gelatine plates, where 

 it forms small round 'or oval colonies of a brownish 

 colour. It is in shape what the French call batonnet; 4 

 and Afanassieff, who injected pure cultures of this 

 microbe into the trachea of dogs and cats, has produced 

 the typical symptoms of whooping cough in these 

 animals. 



1 Deutuche Medicinische Wochenschrift, 1887, p. 1033. 



2 Society de Me'decine Interne de Berlin, 1887. 



3 St. Petersburger Medicinische Wochenschrift, 1887. 

 * Pointed at both ends, like a tip-cat. 



