758 HISTOLOGIG TECHNIC 



11. Dry with blotting paper, drop in absolute alcohol, pass 

 through bergamot oil into xylol and mount in balsam. 



According to Wildman (Jour. Morph., 24, 3, 1913) the slides may 

 be passed from the alizarin solution after rinsing, 1, into a 3 per cent, 

 solution of crystal violet (3 c.c. anilin stain in 100 c.c. distilled water) 

 for ten minutes; 2, rinse and place in 80 per cent, alcohol for five sec- 

 onds; 3, pass through 95 per cent, and 100 per cent, alcohol, where the 

 differentiation is watched; 4, clear and mount in the usual way. 



III. EEGAUD'S METHOD (Arch. d'Anat. Micr., t. 11, 1910, p. 296). 

 This constitutes a very satisfactory routine technic. 



1. Fix small piece of tissue for twenty-four hours in a mixture of 

 Potassium bichromate, 3 per cent, aqueous solution. . 80 parts 

 Formalin 20 parts 



2. Transfer to a 3 per cent, aqueous solution of potassium bichro- 

 mate. This acts as a mordant. The tissue should be left in this solu- 

 tion for from 1 to 4 weeks, with several changes of fluid. 



3. Wash, harden, embed in paraffin, and cut sections of from 3 to 

 5 microns. 



4. Stain with iron-hematoxylin. 



Mallory's Phosphotungstic Acid Hematoxylin for Neuroglia and 

 may be used to advantage with the connective tissues) (Jour. Exp. 

 Med., Vol. 19, No. 5, 1900). Tissues should be fixed in Zenker's fluid 

 or in Kelly's fluid. It stains neuroglia, myoglia and fibroglia fibrils 

 and fibrin and nuclei blue; collagen fibrils are stained reddish-brown; 

 other intercellular substances pink. 



Hematoxylin 0.1 gram 



Water 80.0 c.c. 



Phosphotungstic acid (Merck), 10 per cent. 



aqueous solution 20.0 c.c. 



Dissolve the hematox3'lin by heating in a small amount of water; 

 when cool add to rest of solution. Allow to ripen several weeks. The 

 solution can be ripened for immediate use by addition of 10 c.c. of 14 

 per cent, solution of potassium permanganate, or by the addition of 

 0.2 gram peroxid of hydrogen. 



1. Pass sections through graded alcohols to water. 



2. Place in 14 per cent, aqueous solution of potassium perman- 

 ganate for five to ten minutes. 



