DIRECTIONS FOB LABORATORY WORK 779 



muscle, showing epimysium, muscle fasciculi and enveloping perimy- 

 sium, individual fibers and enveloping endomysium. 



(6) Study an injected specimen of skeletal muscle in a stained longi- 

 tudinal section. Draw (100) a few fibers showing the abundant distri- 

 bution and intimate disposition of capillaries and arterioles. 



(D) TENDON. 



(1) Study a stained transverse section of a tendon. Note epitendi- 

 neum, peritendineum enveloping the tendon fasciculi, and the endo- 

 tendineum. Make a sketch of same under the low power (101). Sketch 

 (102) h.p. small area of a transversely cut tendon bundle. Note the 

 shape of the tendon cells and their relation to the smallest subdivisions 

 (primary bundles) of the fasciculi. Sketch (103) two adjacent tendon 

 fasciculi, from a stained longitudinal section. Note peritendineum; the 

 disposition of the cells with respect to the tendon bundles, and to each 

 other; and the disposition of the nuclei in successive cells. Genetic 

 relationship between tendon and muscle? 



(2) Study the area of junction between muscle and tendon, from a 

 stained longitudinal section (e.g., leg muscle of frog). Sketch (104). 

 What is the relation of the tendon fibrils to the muscle fibrils? to the 

 sarcolemma? Note the increased abundance of nuclei at this level. 

 Significance? Two chief modes of muscle-tendon connections? 



(3) Sketch from specially prepared demonstration slides: (a) 

 neuromuscular (sensory) end organ, or muscle spindle (105) ; (d) 

 neurotendinous (sensory) end organ, or tendon spindle (106) ; (c) 

 motor end plate (107). 



VIII. NERVOUS TISSUE 



(A) THE NEURON (Nerve cell). 



(1) Study under cover-glass an isolated nerve cell (neurocyte) from 

 a macerated preparation, or a teased fresh specimen, of the gray matter 

 of the spinal cord of the ox. Add a drop of stain. Sketch (108) a com- 

 plete cell, noting axon, dendrons, axon hillock (implantation cone), 

 nucleus, nucleolus, and granular and fibrillar cytoplasmic contents. 

 What are the marks of differentiation between an axon and the den- 

 drons ? 



(2) Sketch (109) a similar cell from a stained transverse section 

 of the spinal cord. 



