DISSECTION OF SMALL. NEMATOCERA 419 



Phlebotomus ... Professor Newstead, F.R.S., School of Tropical 

 Medicine, Liverpool. The Indian species should 

 be sent to Dr. Annandale, Indian Museum, 

 Calcutta. 



Culicinae ... Mr. F. V. Theobald, Wye Court, Wye, Kent. Or 



the Central Malaria Bureau, Kasauli, Punjab, 

 India. 



Muscidae ... Professor Bezzi ; Mr. Austen, British Museum 



(Natural History), South Kensington, London. 



Tabanidae ... Miss Ricardo, British Museum (Natural History); 



Baron J. M. R. Surcouf, Museum National 

 D'histoire Naturelle de Paris, France. 



Pupipara ... Dr. P. Speiser, Kgl. Kreisartz, Labes, Pommern, 



Germany. 



DISSECTION 



The dissection* of dipterous insects does not usually present much dif- 

 ficulty, if the task is approached with some knowledge of the anatomy 

 of the parts. Those usually required for examination, the alimentary 

 tract, salivary glands, and ovaries, can be drawn out by traction, the 

 mid-gut, hind-gut and ovaries through the posterior end of the abdomen, 

 the anterior part of the alimentary tract and the salivary glands through 

 the neck. 



In all cases the wings and legs should be removed before commencing 

 the dissection, and if possible the latter should be torn off at the junction 

 with the thorax. The removal of the appendages may be carried out 

 with the fingers or with fine scissors and forceps ; in the case of small hairy 

 or scaly insects the vestiture should be removed as far as possible by 

 means of a soft brush. The debris of appendages and vestiture must on 

 no account be allowed to fall on the slide on which the dissection is to 

 be carried out, or it will cause trouble by adhering to the soft parts. 



The following method will serve for the dissection of the posterior 

 part of the alimentary canal and the reproductive organs from small 



Nematocera, such as Phlebotomus, Siiniilitun, and the 



. . ... . . Small Nematocera 



Culicidae. Place the trimmed insect on a slide with 



the posterior end of the abdomen towards you and just touching the 



* A general account of the technique of dissection, with description of the apparatus 

 required, will be found in Chapter XI, which is intended as an introduction to the de- 

 tailed methods described under each group. 



