oar 



leucocythaemic blood, and in the muscles, ligaments, and articula- 

 tions of pigs with guanin gout. 



Guanin is a colorless, ordinarily amorphous powder which may 

 be obtained as small crystals by allowing its solution in concentrated 

 ammonia to spontaneously evaporate. It is nearly insoluble in 

 water, alcohol, and ether. It is easily dissolved by mineral acids 

 and alkalies, but it dissolves with great difficulty in ammonia. 

 The silver combination dissolves with difficulty in boiling nitric 

 acid, and on cooling the double combination crystallizes. Guanin 

 acts like xanthin in the nitric-acid test, but gives with alkalies on 

 heating a stronger bluish-violet color. A warm solution of guanin 

 hydrochloride gives with a cold saturated solution of picric acid 

 a yellow precipitate consisting of silky needles (CAPRANICA). With 

 a concentrated solution of potassium chromate a guanin solution 

 gives a crystalline, orange-red precipitate, and with a concentrated 

 solution of potassium ferricyanide a yellowish brown, crystalline 

 precipitate (CAPRANICA). 



Adenin, C 6 H 6 N 6 , was first found by KOSSEL in the pancreas 

 gland. It occurs in greatest quantities in the sperm of the carp 

 and in the thymus (KOSSEL and SCHMIDLER). It also occurs in 

 the liver, spleen, lymphatic glands, and kidneys (not in muscles). 

 It has been observed in leucocythaemic urine. 



Adenin crystallizes in long needles. It dissolves in cold water 

 with difficulty (1086 parts), but easily in warm. Pure adenin dis- 

 solves slightly in boiling alcohol, in cold not at all ; impure adenin 

 is, however, dissolved by cold alcohol. It is insoluble in ether. 

 Adenin is easily dissolved by acids and alkalies. In dilute ammo- 

 nia it dissolves with more difficulty than hypoxanthin, but more 

 easily than guanin. The silver combination dissolves with difficulty 

 in boiling nitric acid and crystallizes on cooling. The nitric-acid 

 test and WEIDEL'S reaction act the same as with hypoxanthin. 

 The same is true for its behavior with hydrochloric acid and zinc 

 with addition of alkali. 



The principle for the preparation, detection, and the quantitative 

 estimation of the four above-described xanthin bodies is, according to 

 KOSSEL and KOSSEL and SCHINDLER, as follows : The finely-divided 

 organ or tissue is boiled for three or four hours with sulphuric acid 

 of about 5 p. m. The filtered liquid is freed from albumin by lead 



