THE BLOOD. 67 



To isolate the stromata of the blood-corpuscles they are washed 

 first by diluting the blood with 10-20 vols. of a 1-2$ common-salt 

 solution and then separating the mixture by centrifugal force or 

 by allowing it to stand at a low temperature. This is repeated a 

 few times until the blood-corpuscles ar# freed from serum. These 

 purified blood-corpuscles are, according to WOOLDRIDGE, mixed 

 with 5-6 vols. of water and then a little ether is added until com- 

 plete solution is obtained. The leucocytes gradually settle to the 

 bottom, a movement which may be accelerated by centrifugal force, 

 and the liquid which separates therefrom is very carefully treated 

 with a 1$ solution of KHS0 4 until it is about as dense as the 

 original blood. The separated stromata is collected on a filter and 

 quickly washed. 



WOOLDRIDGE found as constituents of the stroma lecithin, 

 vholesterin, and a globulin which, according to HALLIBURTON, is 

 the above-mentioned (page 58) fibrinoplastic acting cell-globulin. 

 Nucleo-albumin and albumoses could not be detected (HALLI- 

 BURTOK). The nucleated red blood-corpuscles of the bird con- 

 tain, according to PLOS'Z and HOPPE-SEYLER, nuclein and an al- 

 buminous body which swells to a slimy mass in a 10$ common-salt 

 solution and which seems to be closely related to the hyaline sub- 

 stance (hyaline substance of KOVIDA) occurring in the lymph-cells. 

 The red blood-corpuscles without any nucleus are, as a rule, very 

 poor in albumin but are rich in haemoglobin; the nucleated cor- 

 puscles are richer in albumin and poorer in haemoglobin. 



A gelatinous, albuminous, fibrin-like body may be obtained from 

 the red blood-corpuscles. This fibrin-like mass has been observed 

 on freezing and then thawing the sediment of the blood-corpuscles, 

 or on discharging the spark from a large Leyden jar through the 

 blood, or on dissolving the blood-corpuscles of one kind of ani- 

 mal in the serum of another (LANDOIS, stroma fibrin). In none 

 of these cases has it been shown that we have to deal with a fibrin 

 formation at the expense of the stroma. It seems only to have 

 been shown that the red blood-corpuscles of frog's blood contain 

 fibrinogen (ALEX. SCHMIDT and SEMMER). 



The mineral bodies of the red corpuscles -are chiefly potassium, 

 phosphoric acid, and chlorine ; in the red corpuscles of man, dog, 

 and the ox sodium has also been found. 



