106 



PHYSIOLOGICAL CHEMISTRY. 



Another method, suggested by HOPPE-SEYLER, is to determine 

 the total amount of haemoglobin and albumin in a portion of blood, 

 and on the other hand the amount of haemoglobin and albumin in 

 the blood-corpuscles of an equal portion of the same blood which 

 have been sufficiently washed with common-salt solution by centrif- 

 ugal force. The figures obtained as a difference between these two 

 determinations correspond to the amount of albumin which was 

 contained in the serum of the first portion of blood. If we now 

 determine the albumin in a special portion of serum of the same 

 blood, then the amount of serum in the blood is easily determined. 

 The usefulness of this method has been confirmed by BUNGE by 

 the control experiments with the sodium determinations. If the 

 amount of serum and blood-corpuscles in the blood is known, and 

 we then determine the amount of the different blood-constituents 

 in the blood-serum on one side and of the total blood on the 

 other, the distribution of these different blood-constituents in the 

 two chief components of the blood, plasma and blood-corpuscles 

 may be ascertained. According to the just-mentioned procedure, 

 the following analyses of pig's blood and ox's blood have been made 

 by BUNGE. Analyses of human blood have been made for some 

 time by C. SCHMIDT according to another method, which perhaps 

 have given rather too high results for the weight of the blood- 

 corpuscles. All figures represent parts in 1000 parts of blood. 



HOPPE-SEYLER, SACHARJIX, and OTTO found 584.9-693.5 p. m. 

 plasma and 415.1-306.5 p. m. blood-corpuscles in horse's blood. 

 BUNGE found, on the contrary, in an analysis 468.5 p. m. serum 

 and 531.5 p. m. blood-corpuscles more blood-corpuscles, therefore, 



