136 PATHOGENIC BACTERIA. 



is u much the same as if one would inject through a fire- 

 hose three or four quarts of some liquid into the respira- 

 tory organs of a man. ' ' 



The blood of animals, when it is necessary to experi- 

 ment with it, is best secured from 

 a large vein, generally the jugu- 

 lar. From small animals, such as 

 guinea-pigs, it may be secured by 

 introducing a small cannula into 

 the carotid artery. 



Our observations of animals by 

 no means cease with their death. 

 Indeed, he cannot be a bacteriol- 

 ogist who is not already a good 

 pathologist and expert in the recog- 

 nition of diseased organs. 



When an autopsy is to be made 

 upon a small animal, it is best to 



FIG. 3 6.-Mouse-holder. wash * for a few moments in a 

 disinfecting solution, to kill the 



germs present upon the hair and the skin, as well as to 

 moisten the hair and enable it to be kept out of the 

 incision. 



The animal should be tacked to a board if small, or 

 tied, by cords fastened to the legs, to the corners of a 

 table if large, and should be dissected with sterile knives 

 and scissors. When a culture is to be made from the 

 interior of an organ say the spleen it should be incised 

 deeply with a sterile knife and the culture made from 

 its centre. 



Fragments intended for subsequent microscopical ex- 

 amination should be cut very small (cubes of i c.cm.), 

 placed in absolute alcohol for a few hours, then trans- 

 ferred to weaker alcohol, 80-90 per cent., for preserva- 

 tion. The technique of imbedding and staining the tis- 

 sues can be found in almost any reliable text-book on 

 pathology or on the special subject of microscopical 

 technique. 



