212 Dr. II. Charlton Basllan on tJiQ Hsterogenet'ic 



than above, while in C a similar mass is to be seen whicli has 

 almost completely segmented into Fungus-germs. In E we 

 have two other much smaller masses of Zooglcea which have 

 become brown, and which plainly show their constituent 

 bacteria ; while in F we have the same process taking place 

 in very minute units of Zooglcea, such as give rise to discrete 

 Fungus-germs — though in other cases very similar minute 

 units of Z)ogloea, to which I shall presently refer, are trans- 

 formed either into Monads or into Amoebte. 



These facts concerning the changes taking place in large 

 or small masses of Zooglcea, even leaving aside tiie question 

 of their subsequent transformations, are surely facts of con- 

 siderable im])ortance well worthy of a little attention on the 

 ])art of bacteriologist-;. 



Although my more complete recent observations as to the 

 assumption o£ a brown colour by the Zoogloea masses and 

 their segments serve to make the relationshipbetween them and 

 the brown Fungus-germs more obvious than it was previously, 

 yet the actual proof of the development of t'le one from the 

 other can now be given in a more complete way — also as a 

 result of recent observations. 



(2) TJie Transformation of Zoogloea Masses into Fungus- 

 germs or Monads. — The very distinct specimens to which I 

 shall first refer were taken from the scum on a hay-infusion 

 that had been exposed in a small closed pot to a tempera- 

 ture of 70° F. (21° C.) for seven days. Many of the Zoogloea 

 masses had by that time become wholly transformed into 

 brown Fungus-germs, though other masses in all inter- 

 mediate stages were to be seen. Some were still in an early 

 colourless state, as in fig. 1, A, the mass there represented 

 having been taken from this infusion. A later stage in 

 which the whole mass is being converted into embrj^o Fungus- 

 germs is shown in fig. 3, A ; and a still later stage, in which 

 the nucleated embryo germs are far more distinct, is shown 

 in B. In C the germs may be seen to be larger and becom- 

 ing brown, while in D (shown under a lower magnification) 

 the entire mass of the Zoogloea has been transformed into 

 aggregates of brown Fungus-germs. 



In the pot from which these specimens were taken at the end 

 of the severith day not a single hypha was to be found, nor 

 were there any of the thick-walled brown cells to which 

 Mr. Massee refers^. All such bodies were similarly absent 

 fur many days after, during which from time to time I 



* '>'atiue,' Dec. ±2, in04. p. i;."). 



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