522 THE CHL'MlsTh') OF TLMORS 



found that the serum of rabbits immunized with Bence-Jones protein 

 gives the precipitin reaction witli human serum, is evidence that the 

 protein is a human tissue protein and not merely an absorbed and 

 excreted food protein. This has been corroborated by Hopkins and 

 Savorj-,*'^ who also found that the amount of protein in the urine, 

 which contained about one-third the total nitrogen excreted, varied 

 with the general metabolism and was not controlled by the diet. 

 i\Iassini "^^ reports securing positive complement fixation tests with 

 immune sera, differentiating the Bence-Jones protein from normal 

 serum proteins; positive sensitization tests were not obtained by cu- 

 taneous injections of the protein by Boggs and Guthrie. Injected into 

 the blood it is non-toxic and does not lower coagulability as a proteose 

 would. It is capable of acting as an antigen in anaphylaxis reactions, 

 wliich also indicates that it is a complete protein and not a cleavage 

 product.®" When injected into dogs it is partly utilized, although 

 nephritic animals excrete it partly hydrolyzed into proteose.*'-^ 



67 Corroborated also bv Bosprs and Guthrie, Amer. Jour. Med. Sci., 1012 (144), 

 803; Folin and Denis, Jour. Biol. Chem.. 1014 (18), 277. 

 esDeut. Arch. klin. Med., 1911 (104). 20. 

 69Tavlor and Miller, Jour. Biol. Chem.. lOlG (25). 281. 



