116 MICROSCOPIC METHODS 



(b) The following is Neisser's modified cresoidin method : 



1. Stain films for a few seconds in a mixture of solutions A and B, 



two parts of the former to one of the latter. 



A. Methylene-blue . . . . 1 part. 

 Absolute alcohol . '. . . 50 parts. 

 Glacial acetic acid . .. '". . 50 ,, 

 Distilled water .; . . . . 1000 



B. Crystal-violet (Hochst) . ; , 1 part. 

 Absolute alcohol . . . 10 parts. 

 Distilled water -, . .. . 300 ,, 



2. Wash in water, and 



3. Stain in cresoidin solution (1 : 300) for a few seconds (the cresoidin 



should be dissolved in warm water and the solution then 

 filtered). 



4. Wash in water, dry, and mount. 



Sabouraud's Method for Staining Trichophyta. Remove the fat from 

 the hair or epithelial squames witli chloroform. Place in a test-tube 

 with 10 per cent, formol, and warm for two or three minutes till ebullition 

 commences. Wash well in distilled water, and stain for one minute in 

 Sahli's blue, which is made up as follows : 



Distilled water . . . . . .40 parts. 



' Saturated watery methylene blue . . . 24 ,, 

 5 per cent, solution of borax in water . 16 ,, 



Mix the constituents. Allow to stand for a day, and filter. After 

 staining, wash in water, dehydrate with absolute alcohol, clear in xylol, 

 and mount in balsam. 



