258 



GONORRHOEA AND SOFT SORE 



In this position it is usually arranged in chains, which may be 

 of considerable length, and w^hich are often seen lying in parallel 

 rows between the cells. The bacilli chiefly occur in the free 

 condition, but occasionally a few may be contained within 

 leucocytes. 



There is no doubt that in many cases the organism is present 

 in the buboes in a state of purity ; it has been found there by 

 microscopic examination, and cultures have also been obtained 



from this source. The 

 negative results of some 

 observers are probably 

 due to the organism 

 having died off. On the 

 whole the evidence goes 



>* w fJJff F\ to show tliat tlie ordinary 

 (flHllH^' KB J bubo associated with soft 

 * i^JjJpBJr Kl fm sore is to be regarded as 

 ^& ^jlgiKp ^ another lesion produced 



*^ * by Ducrey's bacillus. 



Sometimes the ordinary 

 pyogenic organisms be- 

 come superadded. 



This bacillus takes up 

 the basic aniline stains 

 FIG. 75. Film preparation of pus from soft fairly readily, but loses 

 chancre, showing Ducrey's bacillus, chiefly t ^ Pn lnnr vprv ranirllv 

 arranged in pairs. Stained with carbol- l _ ve !7 ia P lcll > 



fuchsin and slightly decolorised, x 1500. when a decolorising agent 



is applied. Accordingly, 



in film preparations when dehydration is not required, it can 

 be readily stained by most of the ordinary combinations, though 

 Loffler's or Kiihne's methylene-blue solutions are preferable, as 

 they do not overstain. In sections, however, great care must 

 be taken in the process of dehydration, and the aniline-oil 

 method (vide p. 100) should be used for this purpose, as alcohol 

 decolorises the organism very readily. A little of the methylene- 

 blue or other stain may be with advantage added to the aniline- 

 oil used for dehydrating. 



Cultivation. Although for a long period of time attempts 

 to obtain cultures were unsuccessful, success has been attained 

 within recent years. Bezancon, Griffon, and Le Sourd obtained 

 pure cultures in four cases, the medium used being a mixture of 

 rabbit's blood and agar, in the proportion of one part of the 

 former to two of the latter. The blood is added to the agar in 

 the melted condition at 45 C., and the tubes are then sloped. 



