APPEARANCES OF CULTUKKs 



359 



(!'!::. 108). They are more numerous, longer, and more wavy 

 than those of the b. coli. 



Characters of Culture. Generally speaking, on artificial 

 media growths of the b. typhosus appear less dense than 

 those of the b. coli. Stab cultures in peptone gelatin give 

 a somewhat characteristic appearance. On the surface of the 

 medium growth spreads outwards from the puncture as a thin 

 leaf-like film or pellicle, with irregularly wavy margin (Fig. 



FIG. 108. Typhoid bacilli, from a young culture on agar, showing 



Hagella. See also Plate III., Fig. 20. 

 Stained l>y Van Ennengem's method, x 1000. 



109, A). It is semi-transparent and of bluish-white colour. 

 Ultimately this surface growth may reach the wall of the tube. 

 Not infrequently, however, the surface growth is not well 

 marked. Along the stab there is an opaque whitish line of 

 growth, of finely nodose appearance. There is no liquefaction 

 of the medium, and no formation of gas. In stroke cultures 

 there is a thin bluish-white film, but it does not spread to such 

 an extent as in the case of the surface growth of a stab culture 

 (Fig. 109, B). In gelatin plates also the superficial and deep 



