METHODS OF DIAGNOSIS 493 



have given results analogous to those obtained in typhoid 

 (p. 372). 



The Commission has found that vaccination with dead cultures 

 of the micrococcus confers a certain degree of protection amongst 

 those exposed to the disease. As a rule two injections were 

 made, 200-300 million cocci being the dose of the first injection, 

 and about 400 million the dose of the second. The use of vaccines 

 has also been carried out in the treatment of the disease, but 

 the observations are not sufficiently numerous to allow a definite 

 Muit'iiM'iit to be made as to its value. 



Methods of Diagnosis. During life the readiest means of 

 diagnosis is supplied by the agglutinative test just described 

 (for technique, vide p. 118). 



Cultures are most easily obtained from the spleen either 

 during life or post mortem. Inoculate a number of agar tubes 

 by successive strokes and incubate at 37 C. Film preparations 

 should also be made from the spleen pulp and stained with 

 carbol-thionin-blue or diluted carbol-fuchsin (1 : 10). Cultures 

 may sometimes be obtained from the blood by the usual 

 methods. 



