EXPERIMENTAL. 25 



The filtrate from the first precipitation of the substance of preparation 1 1 , 

 caused by saturating its solution with sodium chloride, as described above, 

 was diluted with water and saturated with ammonium sulphate. The pre- 

 cipitate which resulted was dissolved in water and its solution precipitated 

 by saturating with sodium chloride. Although this substance had previ- 

 ously been soluble in saturated brine, after precipitation with ammonium 

 sulphate it was found to be nearly all insoluble therein, so that almost com- 

 plete precipitation resulted on again saturating with sodium chloride. The 

 precipitate so produced was filtered out, dissolved in dilute sodium chloride 

 solution, and reprecipitated by dialysis. We thus secured 7.6 grams of 

 preparation 12. 



By saturating another aqueous extract of germ meal with sodium chloride 

 a very large quantity of protein was separated, which was filtered out, ex- 

 hausted with dilute sodium chloride solution, and the insoluble part washed 

 thoroughly with water and alcohol. This preparation, 13, weighed 17 

 grams. 



The filtrate and saline washings from preparation 13 were united and again 

 saturated with sodium chloride and yielded a small precipitate, which, when 

 dissolved in brine and precipitated by dialysis, gave preparation 14, weighing 

 2.8 grams. As the salt-saturated solution from which this preparation had 

 separated contained so little protein, it appears that nearly all the protein 

 precipitated from the aqueous extract by saturating with sodium chloride had 

 been converted into the insoluble substance forming preparation 13. 



The filtrate from the salt-saturated precipitate produced in the aqueous 

 extract was dialyzed in water for several days, and the still clear solution 

 then dialyzed in alcohol for 24 hours. The protein thereby precipitated in 

 a coagulated state yielded 12.4 grams of preparation 15. 



Another aqueous extract was saturated with sodium chloride, and the 

 precipitate, treated in the same way as preparation 13, yielded 18 grams 

 of preparation 16. 



The saline washings of the last preparation were dialyzed free from chlo- 

 rides and gave a precipitate weighing 2.86 grams, which formed preparation 

 17, having the properties of a globulin, dissolving readily on adding sodium 

 chloride, and being precipitated from such solution by water. 



The filtrate from the final precipitation of 17, when heated in a boiling 

 water-bath, gave a coagulum, which formed preparation 18, weighing 1.64 

 grams. 



The salt-saturated filtrate from the first precipitation of 16, as already 

 described, was heated to boiling, and the coagulum produced was filtered out, 

 giving preparation 19, weighing 5.47 grams. 



Since analysis showed that most of the preparations already described 

 contained phosphorus, some even in large amount, we made an attempt to 



