EXPERIMENTAL. 



39 



The filtrate and washings obtained from this preparation after the second 

 precipitation by dialysis were precipitated by adding a few drops of sodium- 

 chloride solution. The precipitate produced, which was nearly free from 

 coloring matter, weighed 1.25 gram. When analyzed it gave the results 

 shown under the heading " Preparation 26" in the accompanying table. 



Preparations 25, 26, and 27. 



The solution filtered from this preparation was next heated to boiling 

 and the coagulum obtained, which weighed i gram, when analyzed was found 

 to contain the same amount of nitrogen as the globulin. It was considered 

 to be a coagulated globulin which had been held in solution in water by 

 acid. Its nitrogen content is shown under the heading " Preparation 27." 



The total quantity of globulin obtained from the shorts was in this case 

 4.47 grams, being nearly twice as much as was similarly obtained from a 

 like quantity of the flour. 1 



TABI,E 6. Summary of analyses of wheat globulin. 



teller (Bull. 42, Ark. Agr. Exp. Sta., 1896) found a much larger quantity of leucosin 

 and globulin nitrogen in the samples of bran which he examined than he found in the 

 flours. 



