42 THE; PROTEINS OF THE; WHEAT KERNEX. 



filter, dissolved in sodium- chloride solution, and precipitated by water, giving 

 15.4 grams of preparation 34. 



To determine the quantity of globulin contained in the oil-free germ meal, 

 200 grams of the meal were treated with 2000 cc. of 3 per cent sodium- 

 chloride solution heated to 65 and the extract filtered perfectly clear. Of 

 this, 1000 cc. were dialyzed until free from chlorides, when the precipitate of 

 spheroids was filtered out. This preparation, 35, formed 5.05 per cent of 

 the oil-free meal. 



To obtain a quantity of this globulin for digestion with pepsin, a quantity 

 of germ meal was extracted with 3 per cent sodium-chloride solution heated 

 to 70; the extract was filtered clear and saturated with ammonium sul- 

 phate. The precipitate produced was dissolved in water and the resulting 

 gummy and somewhat turbid solution filtered clear. The filtrate was dia- 

 lyzed until the solution gave no turbidity on pouring into distilled water. 

 The protein, which had then separated in spheroids, weighed 27.3 grams, 

 preparation 36. 



A part of the extract from which 36 had been prepared was mixed with an 

 equal volume of decinormal potassium hydroxide solution about twice the 

 quantity necessary to neutralize the extract to phenolphthalein. The solu- 

 tion was then dialyzed in distilled water frequently renewed, and in this way a 

 considerable quantity of phosphorus was separated in the alkaline dialysate. 

 When all, or nearly all, which it was possible to separate in this way had 

 been removed, the solution in the dialyzer was neutralized with hydrochloric 

 acid until it no longer reacted alkaline to litmus. This caused a turbidity. 

 The acid was then further added until an acid reaction with litmus was 

 obtained, producing a precipitate from which, after settling, the solution was 

 decanted. The precipitate was then dissolved in sodium-chloride solution, 

 its -solution filtered clear and dialyzed, whereby a substance was precipitated 

 in spheroids, which formed preparation 37, weighing 3.0 grams. These 

 preparations had the composition shown in table 7. 



TABLE 7. Composition of preparations extracted by sodium-chloride solution 

 from the wheat embryo. 



