EXPERIMENTAL. 



61 



colored mother-liquor was decanted and this process repeated. The pre- 

 cipitated protein was then again dissolved in a little dilute alcohol, and the 

 resulting solution poured into absolute alcohol, thereby precipitating the 

 greater part of the protein and leaving the alcohol strongly colored. The 

 precipitate was thus freed from a very considerable part of its coloring 

 matter. After digestion with absolute alcohol, and finally with ether, this 

 preparation, 80, was dried and analyzed with the result shown in the 

 table below : 



Owing to the fact that this preparation, 80, was still contaminated with 

 coloring matter, and also showed slight differences in composition from the 

 protein extracted by similar treatment from the flour, it was subjected to 

 further treatment with a view to its more complete purification. 



Apart of the preparation was dissolved in isocc. of alcohol of 0.90 sp. gr. 

 and the solution poured into 1000 cc. of absolute alcohol. This produced a 

 turbid liquid, which, on adding a drop or two of sodium-chloride solution, 

 gave a heavy precipitate that rapidly settled, leaving the alcohol colored 

 yellow. This precipitate was again dissolved in diluted alcohol, and pre- 

 cipitated by pouring into ether in order to remove anything soluble in this 

 liquid. No coloring matter was thus removed. The precipitate was then 

 digested with absolute alcohol, yielding preparation 81. 



The strong alcoholic solution from which this preparation had separated 

 on longer standing deposited a small amount of substance which, when 

 dehydrated, yielded preparation 82. Analysis showed these two substances 

 to have the composition shown in the following table : 



Preparations 80, 81, and 82. 



These figures show that the protein extracted from the " shorts " has the 

 same composition as that similarly obtained from the flour. 



