84 PROTEIN POISONS 



follows: glutamic acid, 3 per cent.; glycocoll, 0.33 per cent.; 

 alanin, 1 per cent.; valin, 1.6 per cent.; leucin, 2 per cent.; 

 and phenylalanin, 0.2 per cent. 



The cellular protein of the bacillus coli communis with 

 which we did this work contained 13.74 per cent, of moisture 

 and 7.2 per cent, of ash, or 8.38 per cent, of ash in the 

 moisture-free substance. 



Hydrolysis of the Cellular Protein of the Bacillus Tuber- 

 culosis. Five hundred grams of this substance was hydro- 

 lyzed after the manner already given. The air-dried humus 

 from this substance weighed 120 grams or 24 per cent, of 

 the cellular substance, or 27 per cent, of the moisture-free 

 substance. The humus was found to contain 14.34 per cent, 

 of moisture, 0.15 per cent, of ash, and 1.69 per cent, of 

 nitrogen. 



Before hydrolyzing this substance samples were taken, 

 and the following determinations made. 



Percentage of moisture 12.07 



Percentage of ash . 7 . 08 



Percentage of ash in moisture-free substance 8.05 



Nitrogen was found distributed as follows: 



Percentage of nitrogen in cell substance 9 . 27 



Percentage of nitrogen in moisture-free cell substance . . . . 10.55 



Percentage of nitrogen in ash-free cell substance 9.98 



Percentage of nitrogen in ash- and moisture-free cell substance . 11 .47 



Total amount of nitrogen in 500 grams of cell substance . 46 . 3500 grams 



Total amount of nitrogen in the hydrolyzed fluid . . . 36.3075 grams 



Total amount of nitrogen in the extract of humus . . 4 . 0530 grams 



Total amount of nitrogen in the extracted humus ... 2 . 0388 grams 



Total amount of nitrogen in 500 grams of cell substance . 46.3500 grams 



Total amount of nitrogen in the hydrolytic product . . 42 . 3993 grams 



Total amount of nitrogen lost in hydrolysis .... 3 . 9507 grams 



Amount of nitrogen in hydrolyzed fluid 36 . 3075 grams 



Amount of nitrogen in extract of humus 4 . 0530 grams 



Amount of nitrogen in fluid to be esterified .... 40.3605 grams 



It is thus seen that there is in the fluid to be esterified, 

 40.3605 grams of nitrogen, and it is supposed that this 

 exists in the form of mono- and diamino-acids. 



