BACTERIAL CELLULAR SUBSTANCE 87 



Hydrolysis of the Non-poisonous Portion of the Cellular 

 Protein of the Bacillus Tuberculosis. Five hundred grams 

 of this non-poisonous bacterial split product, known in this 

 laboratory as "residue" or haptophor, was hydrolyzed. 

 The humus was found to constitute 29 per cent, of the air- 

 dried, or 40 per cent, of the moisture-free substance, the 

 percentage of moisture being 27.5. 



The distribution of nitrogen was studied with the fol- 

 lowing results: 



Percentage of nitrogen in residue 4 . 59 



Percentage of nitrogen in moisture-free residue 6 . 34 



Percentage of nitrogen in ash-free residue 5 . 54 



Percentage of nitrogen in ash- and moisture-free residue . . . . 8 . 29 

 Amount of nitrogen in the hydrolyzed fluid .... 18 . 980 grams 

 Amount of nitrogen in the extract of humus .... 1 . 888 grams 

 Amount of nitrogen in the extracted humus .... 2 . 030 grams 



Total 22.898 grams 



Total amount of nitrogen in the 500 grams of residue . 22.990 grams 

 Total amount of nitrogen in the products of hydrolysis . 22 . 898 grams 



Loss during hydrolysis . 092 gram 



Amount of nitrogen in the hydrolyzed fluid .... 18 . 980 grams 

 Amount of nitrogen in the extract of humus .... 1 . 888 grams 





Amount of nitrogen in the fluid to be esterified ... 20 . 868 grams 



No glutamic acid could be obtained from the residue. 

 The result of the fractional distillation is shown as follows : 



Temperature of bath. Yield. 



Fraction I 40 to 60 21. 5 grams 



Fraction II 60 to 80 5.0 grams 



Fraction III 80 to 100 8.0 grams 



Fraction IV 100 to 130 9.0 grams 



Fraction V 130 to 170 10.0 grams 



By the hydrolysis of 500 grams of tubercle residue the 

 following substances were obtained in the amounts and 

 percentages given: 



Per cent, of Per cent, of 



Substance. Amount. residue. dry residue. 



Humus .... 145. 00 grams 29.00 40.00 



Alanin .... 4.00 grams 0.80 1.10 



Leucin . . . . 6 . 40 grams 1 . 28 1 . 76 



Valin .... 2. 30 grams 0.46 0.63 



Phenylalanin . . 1.70 grams 0.34 0.46 



