THE CLEAVAGE OF PROTEINS WITH ALKALI 111 



and showing both Millon and biuret tests, but not seriously 

 affecting animals in usual doses. 



The nitrogen in a number of the crude poisons has been 

 determined by Gidley in this laboratory as follows: 



PERCENTAGE OF NITROGEN IN PROTEIN POISONS. 



Per cent, of N. 



Source of poison. in crude poison. 



Colon bacillus 13.49 



Typhoid bacillus 11.52 



Tubercle bacillus 11.00 



Pyocyaneus 10.50 



Ruber of Kiel 10.495 



Subtilis 8.12 



Megaterium 8.595 



Proteus vulgaris 10.17 



Yellow sarcine 6.145 



Egg albumen (Leach) 13 . 74 



Serum albumin 10.48 



Edestin 12.78 



Zein 10.69 



Witte peptone 11.14 



De Chapoteaut peptone ........ 12.735 



To study the distribution of the nitrogen, determinations 

 were made in both the colon and albumin poisons, of the 

 ammonia nitrogen, the mono-amino, and diamino nitrogen, 

 by the method already described under cleavage with 

 dilute mineral acids. The following are the results: 



DISTRIBUTION OF NITROGEN IN PROTEIN POISONS. 



Total N Mono- 

 Source of Total of acid Ammonia amino Diamino 



poison. poison. extract. N. N. N. 



Colon bacillus . 13.49% 10.185% 1.525% 6.472% 1.753% 



Egg albumen . 13.74% 11.477% 0.745% 7.999% 1.400% 



It will be seen that the greater part of the nitrogen is to 

 be found in mono-amino combination. From the phospho- 

 tungstic filtrates, from both the albumin and colon poisons, 

 containing the mono-amino acids, crystalline bodies were 

 obtained. Judged by the strong Millon test, tyrosin was 



