158 PROTEIN POISONS 



TABLE XV 



These animals had one dose of 50 mg. of the residue, 

 as the non-poisonous portion is designated. The protocol 

 number, the weight of the animal, the interval in days 

 between the administration of the residue and the inocula- 

 tion with the twenty-four hours beef-tea culture of the 

 bacillus, the amount of the culture and the result are shown : 



Protocol, Weight, Interval, Amount given, 



No. gm. days. c.c. Result. 



324 400 1 5 Recovery 



325 420 3 5 Recovery 



269 505 4 3 Recovery 

 275 300 4 5 Recovery 



326 315 7 5 Death 



270 600 9 3 Recovery 

 328 345 11 2 Recovery 

 207 455 21 2 Recovery 



209 495 27 4 Death 



210 625 27 4 Death 



211 485 28 3 Death 



139 240 36 2 Recovery 



140 250 40 3 Death 



141 250 42 2 Death 



142 255 42 2 Death 



In studying these results it will be well to consider the 

 minimum fatal dose as the unit, which in case of the cultures 

 used in these experiments is 1 c.c. of the twenty-four hour 

 beef -tea growth, and we will regard the animal that suc- 

 cumbs to 2 c.c. as having practically lost its immunity. 

 With this measure it will be seen that a single dose of 50 

 mg. of the colon residue gives to the animal a temporary 

 immunity of at least 5 units, which is in force twenty-four 

 hours after the treatment, and continues for at least four 

 days, but has begun to disappear by the seventh day. 

 However, some slight degree of immunity continues up to 

 the thirty-sixth day, but practically all is lost by the 

 fortieth day. 



TABLE XVI 



These animals had a single dose of 25 mg. of the residue. 

 The data are the same as given in the preceding table. 





