254 PROTEIN POISONS 



We have tried to extract the protein poison from Witte's 

 peptone by long-continued shaking with absolute alcohol, 

 but with only negative results. It is probable that the 

 poison as it exists in peptone is in the form of a larger 

 molecule than is split off by our method, and consequently 

 is not soluble in absolute alcohol. 



Passive Anaphylaxis. The serum of a sensitized animal 

 introduced into a fresh animal renders the latter suscep- 

 tible. The second animal may be of the same or another 

 species. In the former case the condition induced by the 

 transference of the serum is known as homologous, and in 

 the second as heterologous passive anaphylaxis. On account 

 of the ease and completeness with which it is sensitized the 

 guinea-pig is most frequently the recipient, whatever be 

 the species of the donor. The transfer of the condition of 

 sensitization from the mother to her offspring is an illus- 

 tration of homologous passive anaphylaxis. This has been 

 studied especially by Rosenau and Anderson, Gay and 

 Southard, and Otto. The latter has found the young 

 sensitive at forty-four days after birth. Gay and Southard 1 

 were the first to demonstrate experimental passive anaphyl- 

 axis. These investigators found their recipients first sensi- 

 tive on the fourteenth day. This indicated a somewhat 

 long period of incubation for the development of the ana- 

 phylactic state in the recipient, and this was not easily 

 explainable. Otto and Friedemann 2 injected the anaphyl- 

 actic serum subcutaneously and the antigen intraperi- 

 toneally twenty-four hours later. With shorter intervals 

 they failed to obtain any response. Braun 3 was the first 

 to inject the anaphylactic serum intravenously, but even 

 with this method a short period of incubation seemed to be 

 necessary. By injecting both sera intravenously, Doerr 

 and Russ 4 cut down the supposed period of incubation to 

 four hours, but their most striking and constant results 



1 Jour. Med. Research, 1907, xvi, 143. 



2 Munch, med. Woch., 1907. 



3 Zeitsch. f. Immunitatsforschung, 1910, iv. 

 Ibid., iii, 181. 



