PROTEIN SENSITIZATION OR ANAPHYLAXIS 263 



the former be subcutaneous, the latter may be intraperi- 

 toneal or intravenous, or vice versa. However, when the 

 animal has been sensitized by several (six to eight) subcu- 

 taneous or intraperitoneal injections, and the reinjection 

 consisting of 2 c.c. is made into the vein, the animal, in 

 many instances, dies from anaphylactic shock. Imme- 

 diately after the reinjection it shakes its head, and evidently 

 becomes anxious. Breathing is frequent, polypnea reaching 

 sometimes as high a,s 200 to 250 respirations per minute. 

 There is expulsion of stool. Immobility, apnea, and exoph- 

 thalmia appear and the animal may die within three minutes 

 after receiving the reinjection. Obduction shows the heart 

 in systole and the blood fluid. 



The rabbits which recover after manifesting disturbances 

 of respiration pass into a long-continued cachectic state. 

 Rabbits which have received daily intravenous injections 

 and then, after a period of time receive the reinjections 

 by the same route, generally, but not invariably, die in 

 anaphylactic shock. Sensitization by repeated intravenous 

 injections and subcutaneous reinjections, invariably result 

 in the production of the Arthus phenomenon. 



Nicolle 1 found that rabbits in which Arthus' phenomenon 

 has been developed become highly receptive to, and readily 

 succumb to, infection. The same investigator produced 

 the Arthus reaction in guinea-pigs, though he found these 

 animals less suitable than rabbits for the demonstration 

 of this form of anaphylaxis. Lewis 2 also demonstrated 

 in guinea-pigs the phenomenon of Arthus. Remlinger 3 has 

 done similar work. He injected massive doses (10 c.c.) 

 of horse serum, at intervals of one week, from six to eight 

 times, and one month after the last, gave a reinjection. 

 On doing this his animals either developed general symp- 

 toms or passed into a cachectic condition and died. Vaughan 

 and Wheeler 4 killed guinea-pigs by daily intraperitoneal 

 injections of egg-white. 



1 Ann. d. 1'Institut Pasteur, 1907, xxi, 128. 



2 Jour. Exper. Med., 1908. 



3 Compt. rend, de la Soc. biol., 1907, Ixii, 23. 



4 Jour. Infect. Dis., June, 1907. 



